Diarylether-based fluorogenic probes for detection of hypochlorous acid
- Research Tools and Methods
Background and Market Opportunity
- 2,7-dichlorofluorescein (DCF) is currently the most successful commercial probe for hypochlorous acid (HOCl), but it suffers from poor selectivity and sensitivity.
- Previous reports have suggested that the probes for HOCl have various limitations in terms of sensitivity, selectivity, chemostability, and photostability toward reactive oxygen species (ROS) and a negligible response toward HOCl.
- Therefore, there is a need for a probe that is highly sensitive, selective, chemo-stable, and photo-stable towards ROS and HOCl.
Technology Overview and Key Advantages
- The present invention describes methods for detecting the presence of and/or determining the level of superoxide, HOCL or hydroxyl radical in samples, or in vivo in an organism.
- The probe exhibits sensitivity and selectivity towards HOCl over a wide range of reactive oxygen and nitrogen species (ROS/RNS) found in cells.
- This probe contains substituted diarylether group as reacting site, which allows it to exhibit escalating sensitivity towards HOCl without any interference from cellular ROS/RNS.
- The performance of these probes is highly robust in multiple cellular models including inflammation and mitochondrial respiratory inhibition.
- Reactive time is very short and detection limit is low, therefore high sensitivity.
- Signal towards hypochlorous acid is high, that is high selectivity.
- Low cytotoxicity.
- Good solubility in aqueous media.
- In vitro and in vivo experimental data is available.
Potential Product and Applications
- The probes holds great potential as a discovery tool for dissecting the roles of HOCl in health and disease.
- The probe could be used for drug screening.
Development Status and IP Strength
- US application: US2016312033 undergoing patent examination.